Fig. 2

Prevalence of [Ni-Fe] hydrogenases orthologous to those in P. thermoglucosidasius among other bacterial taxa. The ML phylogeny was constructed on the basis of the trimmed alignment (1597 amino acids in length) of the concatenated InfB, RecN and RpoB amino acid sequences. Different taxa and branch colours indicate the different phyla/classes. Values on the branches indicate bootstrap values (n = 500 replicates) and the tree was rooted on the midpoint. The presence of [Ni-Fe] group 1d, group 2a and group 4a hydrogenases is represented by dark blue, red and green blocks, respectively. Where [Ni-Fe] hydrogenases belonging to the same groups but not the same subtype as those in P. thermoglucosidasius are present they are indicated by light blue ([Ni-Fe] group 1 hydrogenases), pink ([Ni-Fe] group 2 hydrogenases) and light green ([Ni-Fe] group 4 hydrogenases) blocks, respectively