Table 5 Plasmids used in this study

p414-TEF1p-Cas9-CYC1t

Centromeric plasmid, AmpR, TRP1, TEF1p-Cas9-CYC1t

[22]; Addgene#43802

p426-SNR52p-gRNA.CAN1.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-CAN1.Y

[22]; Addgene#43803

p426-SNR52p-gRNA.X-2.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-X-2.Y

This study

p426-SNR52p-gRNA.XII-2.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-XII-2.Y

This study

p426-SNR52p-gRNA.SPR1.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-SPR1.Y

This study

p426-SNR52p-gRNA.EXG1.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-EXG1.Y

This study

p426-SNR52p-gRNA.TSC13.Y-SUP4t

2 μm ori, AmpR, URA3, gRNA-TSC13.Y

This study

pESC-TRP

Template for A-TRP1-B

Agilent

p424_GPD

2 μm ori, AmpR, TRP1

[57]

pAT423

2 μm ori, AmpR, HIS3

[58]

pF

pAT423, TDH_P-coF3H-TDH_T

This study

pD

pAT423, TDH_P-coGhDFR-TDH_T

This study

p3GT

p424_GPD, PGI1_P-co3GT-CYC_T

This study

pFD

pAT423, ADH_P-coF3H-ADH_T, TDH_P-coGhDFR-TDH_T

This study

pFDA

pAT423, GPD_P-coF3H-TEF_T, TDH_P-coGhDFR-TDH_T, ADH_P-coANS-ADH_T

This study

pFDA3

pAT423, GPD_P-coF3H-TEF_T, TDH_P-coGhDFR-TDH_T, ADH_P-coANS-ADH_T, PGI1_P-co3GT-CYC_T

This study

pUC57 + AtF3H_BN

Template for B-GPD_P-coF3H-TEF1_P-N

Synthetic construct

pUC57 + AtANS_NF

Template for N-TPI_P-coANS-PGK_T-F

Synthetic construct

pUC57 + 2 µm_FG

Template for F-2µm-G

Synthetic construct

pUC57 + GhDFR_GO

Template for G-TEF1_P-coGhDFR-CYC1_P-O

Synthetic construct

pUC57 + MtDFR_GO

Template for G-TEF1_P-coMtDFR-CYC1_P-O

Synthetic construct

pUC57 + AaDFR_GO

Template for G-TEF1_P-coAaDFR-CYC1_P-O

Synthetic construct

pUC57 + At3GT_OI

Template for O-PGI1_P-co3GT-ADH_T-I

Synthetic construct

pUDE172

Template for TDH3_P-atPAL1-CYC1_T, TPI_P-coC4H-ADH_T, PGI_P-coCPR1-PGI_T

[7]

pUDE188

Template for TDH3_P-coCHS3-CYC1_T, TEF1_P-coCHS3-TEF1_P, I-E. coli-A

[7]

pUDI065

Template for TDH3_P-atCHI1-CYC1_T, TPI_P-atCHS3-ADH_T, TEF_P-at4CL3-TEF_T

[7]

pUDI069

Template for TDH3_P-coTAL1-CYC1_T

[7]

pCPW002

2 μm ori, AmpR, TRP1, PGAP1-AtF3H-TTEF1, TPI_P-coANS-PGK_T, TEF1_P-coGhDFR-CYC1_P, PGI1_P-co3GT-ADH_T

This study

pCPW006

2 μm ori, AmpR, TRP1, PGAP1-AtF3H-TTEF1, TPI_P-coANS-PGK_T, TEF1_P-coMtDFR-CYC1_P, PGI1_P-co3GT-ADH_T

This study

pCPW007

2 μm ori, AmpR, TRP1, PGAP1-AtF3H-TTEF1, TPI_P-coANS-PGK_T, TEF1_P-coAaDFR-CYC1_P, PGI1_P-co3GT-ADH_T

This study