Fig. 8

Phenotype of ∆ncw-1∆Ncap3m∆gul-1 triple mutant when grown on Avicel medium. Conidia of the wild type (WT), the double deletion strain (∆ncw-1∆Ncap3m) and the triple deletion strain (∆ncw-1∆Ncap3m∆gul-1) were separately inoculated into Avicel medium, and then cultured for 7 days. a Total extracellular protein concentration, endoglucanase activity, β-glucosidase activity and the viscosity of culture broth were measured and normalized to the WT according to percentage. Values represent the means of at least three biological replicates, error bars show standard deviation. Statistical significance was performed using a two-tailed Student’s t test (***P < 0.001; n.s., not significant). b Mycelial morphologies of the triple deletion strain (∆ncw-1∆Ncap3m∆gul-1) and its parental strain (∆ncw-1∆Ncap3m) after 7 days cultivation. The images were acquired by an Olympus SZX-7 stereomicroscopy with a digital camera attached. Scale bar is 300 μm