Fig. 5

Effect of different concentrations of chemicals on hyphal growth in WT and Δgul-1. Aliquots of 5 μL 1 × 10⁷ mL⁻¹ spore suspensions of WT and Δgul-1 were incubated at 28 °C for 18 h on MM plates incorporating H₂O₂ (10, 20 mM), diamide (6, 9 μg/mL), methyl-viologen (4.5, 9 μg/mL), NaCl (0.5, 1.0 M), Congo Red (1, 2 mg/mL) or Calcofluor White (200, 400 μg/mL), and then the diameter of each colony was measured. Relative diameter reduction means the reduction of growth rate on MM containing chemicals compared with the growth on MM only. Values represent the means of at least three biological replicates, error bars show standard deviation. Statistical significance was performed using a two-tailed Student’s t test (***P < 0.001)