Fig. 3

Comparison of the viscosity of culture broths from WT, Δgul-1, Pc-gul-1 and Pn-gul-1 strains grown on Avicel medium for 7 days. The following strains were grown in 2% (w/v) Avicel media: the WT, the gul-1 gene knockout mutant (Δgul-1) and the complemented strain under either the control of the ccg-1 promoter (Pc-gul-1) or the native promoter (Pn-gul-1). The viscosity was measured and displayed after normalization to the WT control according to percentage. Values represent the means of at least three biological replicates, error bars show standard deviation. Statistical significance was performed using a two-tailed Student’s t test (**P < 0.01; ***P < 0.001; n.s., not significant)