Table 2 Strains and plasmids used in the study
Strains or plasmids | Description | Sources |
|---|---|---|
Strains | ||
 DH5α | E. coli | NEB |
 DH5α/pDXW-8 | DH5α harboring pDXW-8 | This study |
 DH5α/pDXW-8-phaA | DH5α harboring pDXW-8-phaA | This study |
 DH5α/pDXW-8-phaAB | DH5α harboring pDXW-8-phaAB | This study |
 DH5α/pDXW-8-phaCAB | DH5α harboring pDXW-8-phaCAB | This study |
 ATCC13869 | Wild-type C. glutamicum | ATCC |
 ATCC13869/pDXW-8 | ATCC13869 harboring pDXW-8 | This study |
 ATCC13869/pDXW-8-phaCAB | ATCC13869 harboring pDXW-8-phaCAB | This study |
 WM001 | C. glutamicum l-isoleucine producer | CCTCC |
 WM001/pDXW-8 | WM001 harboring pDXW-8 | This study |
 WM001/pDXW-8-phaA | WM001 harboring pDXW-8-phaA | This study |
 WM001/pDXW-8-phaAB | WM001 harboring pDXW-8-phaAB | This study |
 WM001/pDXW-8-phaCAB | WM001 harboring pDXW-8-phaCAB | This study |
Plasmids | ||
 pBHR68 | Template plasmid of phaCAB cluster | [63] |
 pDXW-8 | Shuttle vector between E. coli and C. glutamicum | [27] |
 pDXW-8-phaA | pDXW-8 harboring phaA | This study |
 pDXW-8-phaAB | pDXW-8 harboring phaAB | This study |
 pDXW-8-phaCAB | pDXW-8 harboring phaCAB | This study |