Fig. 1

Distribution of secretory vesicles in both wildtype and hyperbranching (∆racA) backgrounds. Quantification of fluorescence intensity (arbitrary units) by CLSM microscopy of the post-Golgi vesicle marker SncA fused with GFP (a) in the wildtype (FG7), ∆glaA (MF7.4) and GlaA-overexpression (Tet-on-glaA, ∆glaA; MF19.5) strains with 20 µg/mL doxycycline (+DOX); (b) in the hyperbranching GlaA-overexpression strain (Tet-on-glaA, ∆glaA, ∆racA; MF22.4) with or without induction of glaA with DOX. All strains express the GFP-SncA fusion. Fluorescence of vesicles along the hyphae (up to 20 µm from the apex) was quantified from at least 20 hyphae. c Representative pictures (z-stacks) are shown (scale bar ca. 20 μm)