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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Fig. 2

Analysis of the NaCl dependency of αGG synthesis in C. glutamicum (pEKEx2-ggpSP). αGG concentrations in culture supernatants after 24 h cultivation of C. glutamicum (pEKEx2-ggpSP) in minimal medium with initially 1% sucrose as sole carbon source a. NaCl was added in indicated concentrations after 4 h of cultivation. Analysis of cell free extracts (CE) of C. glutamicum (pEKEx2-ggpSP) cultivated at different NaCl concentrations for expression of strep-tagged GgpS by Western immunoblot with antibodies against the tag b; protein sizes (kDa) are indicated for markers (M) bands

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