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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Fig. 1

Heterologous expression of ggpS and ggpP from Synechocystis sp. PCC 6803 enable NaCl-triggerd αGG synthesis in C. glutamicum (pEKEx2-ggpSP). a Analysis of cell free extracts (CE) of C. glutamicum (pEKEx2-ggpSP) for expression of strep-tagged GgpS and his-tagged GgpP by Western immunoblot with antibodies against the tags; protein sizes (kDa) are indicated for markers (M) bands a. Growth (circles) b, substrate consumption (triangles) and αGG fromation (diamonds) c of C. glutamicum (pEKEx2-ggpSP) during cultivation in CgC-minimalmedium with initially 1% sucrose in absence (solid symbols) and presence of 750 mM NaCl (open symbols). NaCl was added to the culture after 4 h of cultivation. Data from one representative experiment of a series of three are shown

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Microbial Cell Factories

ISSN: 1475-2859

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