Fig. 4
Carbon-limited chemostat cultures of S. cerevisiae. a Cell dry weight (CDW; gCDW/L); b intracellular ATP concentration (µmol/gCDW); c intracellular l-methionine concentration (µmol/gCDW); d intracellular SAM concentration (µmol/gCDW). The bold arrows indicate the time points at the start of the carbon-limited chemostat cultures. The dash arrows represent the time points of sampling for intracellular metabolite analysis. Values of intracellular ATP concentration represent the average of five measurements with error bars calculated as the standard deviations of the means. Values of intracellular l-methionine and SAM concentration represent the average of three measurements with error bars calculated as the standard deviations of the means