Table 1 Strains and plasmids used in this study
Names | Descriptions | Sources |
|---|---|---|
Strains | ||
 E. coli WM3064 | RP4 (tra) in chromosome, DAP− | This lab |
 B. animals subsp. lactis | The strain contains xfp gene | BCRC |
 E. coli K12 | The strain contains pta gene | This lab |
 K. robustum SPU_B003 | 2-KGA production strain | This lab |
Plasmids | ||
 pBBR1MCS-2 | Broad host vector | This lab |
 pX551-gfp | Plasmid carries gfp gene | This lab |
 pBBR-PtufB_gfp | Recombinant plasmid carries gfp gene initiated by E. coli_tufB promoter | This study |
 pBBR-P1_gfp | Recombinant plasmid carries gfp gene initiated by P1 (orf_01408) promoter | This study |
 pBBR-P2_gfp | Recombinant plasmid carries gfp gene initiated by P2 (orf_02221) promoter | This study |
 pBBR-xfp-pta | Recombinant plasmid carries xfp and pta gene initiated by their original promoter, respectively | This study |
 pBBR-P1_xfp-P2_pta | Recombinant plasmid carries xfp and pta gene initiated by P1 and P2 promoter, respectively | This study |
 pBBR-P1_xfp2502-P2_pta2145 | Recombinant plasmid carries optimized xfp2502 and optimized pta2145 gene initiated by P1 and P2 promoters, respectively | This study |