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Table 2 Effect of fabH deletion and cerulenin inhibition (1 µg/ml) on DHA producing strains

From: fabH deletion increases DHA production in Escherichia coli expressing Pfa genes

Strain

BW—pDHA4

ΔfabH—pDHA4

Cerulenin

0 µg/ml

1 µg/ml

0 µg/ml

1 µg/ml

Doubling time (h)a

5.3 ± 0.2

6.2 ± 0.2***

7.2 ± 0.2***

8.8 ± 0.7***

%DHA/total FAb

7.5%

15.5%**

23.9%***

31.7%***

mg DHA/lb

2.8 ± 0.4

8.1 ± 0.24***

11.2 ± 1.9***

16.8 ± 1.6***

  1. aMaximum doubling time calculated during the exponential phase for each condition
  2. bDHA production is represented by relative (% DHA/total FA) and absolute (mg DHA per liter of culture) quantification of DHA for each strain and condition
  3. Mean and standard deviation is shown. Asterisks indicate the degree of significance (*** p < 0.0001; ** p < 0.001)
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Microbial Cell Factories

ISSN: 1475-2859

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