Table 1 Strains and plasmids used in this study
Name | Relevant characteristics | Reference or source |
|---|---|---|
Strain | ||
 Escherichia coli JM109 | recA1 endA1 gyrA96 thi hsdR17(r −K m +K ) e14− (mcrA) supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB+ lacIq lacZΔM15] | TakaraBio |
 Corynebacterium glutamicum ATCC13032 | Type-strain of C. glutamicum | ATCC |
 C. glutamicum ATCC31831 | Wild type | ATCC |
 ΔaraR | Markerless araR-deletion mutant of strain ATCC31831 | This study |
 31831/pCH | C. glutamicum ATCC31831 bearing pCH | This study |
 31831/pCHpyk | C. glutamicum ATCC31831 bearing pCHpyk | This study |
 ΔaraR/pCH | ΔaraR bearing pCH | This study |
 ΔaraR/pCHpyk | ΔaraR bearing pCHpyk | This study |
Plasmid | ||
 pCH | Kmr; E. coli–Corynebacterium sp. shuttle vector | [40] |
 pCHgltA | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 gltA gene (NCgl0795) encoding citrate synthase |  |
 pCHpfk | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 pfk gene (NCgl1202) encoding phosphofructokinase |  |
 pCHpyk | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 pyk gene (NCgl2008) encoding pyruvate kinase | This study |
 pK19mobsacB | Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) | ATCC |
 pK19mobsac-ΔaraR | Kanr, pK19mobsacB with the deletion construct for araR of C. glutamicum ATCC31831 | This study |