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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Biosynthesis of angelyl-CoA in Saccharomyces cerevisiae

Fig. 5

Impact of methyl-malonic acid feeding on AN-CoA production. a Graphical representation of the pathway starting with methyl-malonic acid feeding. b Intracellular accumulation of Ac-CoA (orange bars), Pr-CoA (purple bars), MM-CoA (yellow bars), HMB-CoA (red bars) and AN-CoA (green bars) in strains expressing matB (ANG10), ssfNKJ (ANG11) and matB + ssfNKJ (ANG12), upon 12 h of growth. c Time course of production of AN-CoA (open squares), MM-CoA (open circles) and Ac-CoA (filled triangles) in strain expressing matB + ssfNKJ (ANG12), and corresponding optical density at 600 nm (filled circles). All engineered strains were incubated in selective SC medium, supplemented with 0.5 g/L methyl-malonic acid (all data: mean ± SD, n = 3)

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