Fig. 2

Time-dependent expression of RFP using plasmids pF8k-RFP and pF5k-RFP in B. sacchari. Late-exponential cells were diluted 100 times in MMX (with and without inducer) and transferred to 96-well plates. RFP fluorescence and optical density (OD₆₀₀) were measured in triplicate every 15 min for 24 h. IPTG 0.5 mM (solid black line) or arabinose 5 mM (solid gray line) were used as inducers. Fluorescence was normalized by optical density and is expressed in arbitrary units. Uninduced cells are shown as dashed lines. Fluorescence profile of pF5k-RFP (black lines) is plotted on the left Y axis and pF8k-RFP (gray lines) on the right Y axis. All assays were performed in triplicate