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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari

Fig. 1

pF8k-RFP and pF5k-RFP plasmids, two Bglbrick-based expression vectors for B. sacchari. pF8k-RFP (5284 bp) and pF5k-RFP (5617 bp) vectors were constructed by changing the replication origins of BglBrick pB8k-RFP and pB5k-RFP plasmids, respectively. Nomenclature of these plasmids is identical to Bglbrick vectors, except the B (BBR1 from BglBrick vectors) is replaced by F (pBBR1 full from pBBR1MCS-2) (details in text). The figure depicts the origin of replication from pBBR1MCS-2, kanamycin resistance, red fluorescent protein RFP, pBAD expression system including BAD promoter and E. coli araC, placUV5 expression system including lacUV5 promoter and E. coli lacI

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Microbial Cell Factories

ISSN: 1475-2859

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