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Table 1 Strains and plasmids used in this study

From: Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids

Strain or plasmid Relevant characteristics Source or reference
E. coli strains
 DH5α F– Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK–, mK +) phoA supE44 λ– thi-1 gyrA96 relA1 Invitrogen (Karlsruhe, Germany)
C. glutamicum strains
 MB001(DE3) prophage-free derivate of ATCC 13032 with chromosomal expression of T7 RNA polymerase gene under control of PlacUV5 (IPTG-inducible) [65]
 DelAro4 MB001(DE3) derivative with in-frame deletions of cg0344-cg0347, cg2625-cg2640, cg1226 and cg0502 [27]
 DelAro5 DelAro4 derivative with in-frame deletion of cg3349-cg3354 This study
 DelAro5 C7 DelAro5 derivative with an exchange of the native promoter of the citrate synthase gene gltA by the dapA promoter variant C7 This study
 DelAro5 PO6-iolT1 DelAro5 derivative with two point mutations in the promoter of the inositol transporter gene iolT1, abolishes repression of iolT1 by IolR This study
 DelAro5 C7 PO6-iolT1 DelAro5 derivative with combined modifications of DelAro5 C7 and DelAro5 PO6-iolT1 This study
Plasmids
 pMKEx2 kanr; E. coli-C. glutamicum shuttle vector (lacI, PT7, lacO1, pHM1519 ori Cg ; pACYC177 ori Ec ) [65]
 pMKEx2_aroF*_qsuB pMKEx2 derivate for expression of genes coding for AroF* from E. coli (codon-optimized gene) and for QsuB of C. glutamicum (native gene) This study
 pMKEx2_aroF*_irp9 pMKEx2 derivate for expression of genes coding for AroF* from E. coli (codon-optimized) and for Irp9 from Yersinia enterocolitica (codon-optimized) This study
 pMKEx2_aroF*_hyg5 pMKEx2 derivate for expression of genes coding for AroF* from E. coli (codon-optimized) and for Hyg5 from Streptomyces hygroscopicus (codon-optimized) This study
 pMKEx2_aroF*_ubiC pMKEx2 derivate for expression of genes coding for AroF* (codon-optimized) and for UbiC (native gene) from E. coli This study
 pMKEx2_aroH_ubiC pMKEx2 derivate for expression of genes coding for AroH (native gene) and for UbiC (native gene) from E. coli This study
 pEKEx3 specr; E. coli-C. glutamicum shuttle vector (lacI, P tac , lacO1, pBL1ori Cg ; pUCori Ec ) [66]
 pEKEx3_tkt pEKEx3 derivate for overexpression of the transketolase gene tkt of C. glutamicum (the TTG start codon of tkt was replaced for ATG) This study
 pK19mobsacB kanr; vector for allelic exchange in C. glutamicum (pK18 oriV Ec sacB lacZα) [33]
 pK19mobsacB_cg3349-54-updown pK19mobsacB derivate for the in-frame deletion of cg3349-54 This study
 pK19mobsacB_PO6-iolT1 pK19mobsacB derivate for introducing the mutagenized operator sequence into the iolT1 promoter region [48]
 pK19mobsacB_ΔP gltA :P dapA -C7 pK19mobsacB derivate for exchanging the native gltA promoter for the dapA promoter variant C7 [50]
  1. kanr kanamycin resistance, specr spectinomycin resistance