Table 3 Metabolite analysis of culture broths of the K. pneumoniae ΔldhA mutant grown for 24 h by fed-batch fermentation using crude glycerol as a substrate with different medium replacement ratios
Medium replacement ratio (%) | 1,3-PD (g/L) | 2,3-BD (g/L) | Ethanol (g/L) | Acetate (g/L) | Succinate (g/L) | 1,3-PD (g/L/h) |
|---|---|---|---|---|---|---|
5 (1:20) | 68.8 | 30.5 | 6.5 | 2.3 | 8.2 | 2.86 |
10 (1:10) | 79.5 | 39.5 | 6.1 | 1.8 | 11.8 | 3.31 |
20 (1:5) | 78.1 | 40.7 | 7.5 | 1.7 | 12.5 | 3.25 |