Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons

Fig. 3

AD and 4HP bioconversion assays of recombinant strains. LB medium supplied with 1 g L−1 AD or 4HP was used to test the transformation ability of the recombinant E. coli BL21 (DE3) and B. subtilis 6051a strains. BL21-kstD1/BL21-kstD2/BL21-kstD3, E. coli BL21 (DE3) containing pET28a-kstD1/pET28a-kstD2/pET28a-kstD3; BL21-pET28a, E. coli BL21 (DE3) containing empty plasmid pET28a(+); 6051a-kstD1/6051a-kstD2/6051a-kstD3, B. subtilis 6051A containing pHT01-kstD1/pHT01-kstD2/pHT01-kstD3; 6051a-pHT01, B. subtilis 6051a containing empty plasmid pHT01

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us