Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant genotype | Reference |
|---|---|---|
E. coli JM109 |  | Stored in author’s laboratory |
S. cerevisiae BY4741 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | Stored in author’s laboratory |
BY4741 opi1∆ Bga-0 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0, opi1∆0 BY474 carrying pY26GPD-TEF-miox4-udh | Stored in author’s laboratory This study |
Bga-1 | BY474 opi1∆ carrying pY26GPD-TEF-miox4-udh | This study |
Bga-2 | BY474 opi1∆ carrying pY26GPD-TEF-miox4-histag | This study |
Bga-3 | BY474 opi1∆ carrying miox4 and udh | This study |
E. coli pUG6 | E. coli plasmid with segment LoxP-KanMX4-LoxP | Stored in author’s laboratory |
E. coli pSH65 pY26-GPD-TEF pY26-miox4-udh pY26-miox4-histag | Shuttle plasmid for E. coli and S. cerevisiae, Cre gene under GAL2 regulative regulation Integrating plasmid, URA3 marker, MCS derived from pBLUESCRIPT (ColE1 (derivative) ori, f1 ori, AmpR) pY26GPD-TEF carrying miox4 and udh pY26GPD-TEF carrying miox4-histag | Stored in author’s laboratory Stored in author’s laboratory This study This study |