Fig. 3

MIOX4 protein analysis. a Western blot analysis of MIOX4. Whole-cell protein was extracted to perform Western blot after the recombinant strain Bga-2 was cultured in YPD medium with 60 mM (10.8 g/L) myo-inositol for the indicated time. b SDS-PAGE analysis of MIOX4. To purify the MIOX4 protein, the His-tag was fused to the C-terminal of MIOX4. Strains were cultured as above, and the whole-cell protein was extracted and purified with the 6× His-Tagged Protein Purification Kit. The purified protein was verified by SDS-PAGE. Lane 1: protein marker, Lane 2: control (BY4741 carrying pY26 plasmid), Lanes 3 and 4: Bga-2