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Table 2 The half-lives (T1/2) of IDI and IDI (L141H/Y195F/W256C) at different temperatures

From: Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis

Temperature (°C)

IDI

IDI (L141H/Y195F/W256C)

Fold improvement

30

32.7 h (48.9%)

85.0 h (50.1%)

2.6

35

15.3 h (51.0%)

45.6 h (50.7%)

3.0

40

4.0 h (50.3%)

32.9 h (49.8%)

4.7

45

61 min (50.8%)

14.1 h (51.0%)

13.8

50

10 min (49.7%)

2.67 h (49.5%)

16.0

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Microbial Cell Factories

ISSN: 1475-2859

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