Fig. 4
From: A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum
Gene deletion and insertion using the RecET-assisted CRISPR–Cas9 method. a Deletion of ldh gene using pHA500sgRNA ldh and pHA 1000sgRNA ldh . b Deletion of 1-, 10- and 20-kb regions in CGP3 locus using pHAsgRNAΔCGP3–1kb, pHAsgRNAΔCGP3–10kb and pHAsgRNAΔCGP3–20kb. c Gene insertion in upp, CGP1, CGP2, CGP3 loci. For upp locus, both gfp, P tuf -hom-thrB and P tuf -hom-thrB-P glyA -lysC-thrC expression cassettes were chosen as the insertion fragments. For CGP1, CGP2 or CGP3 loci, gfp was chosen as the insertion gene