Fig. 6
From: Gene repression via multiplex gRNA strategy in Y. lipolytica
Multiplex gene interference in Y. lipolytica. a Heterogenous biosynthesis pathway for protodeoxy-violaceinic acid (PVA) production in Y. lipolytica. b Schematic of the plasmid targeting vioA, vioB and vioE simultaneously. Schematic of plasmid targeting both gfp and vioE. c The PVA relative absorbance of strains interfered by dCpf1-Multi and dCas9-Multi system. d The fluorescence and the PVA relative absorbance of strains interfered by dCas9-Multi system. “+” means possess and “−” means not possess. The error bars (mean ± SD) were derived from triplicate experiments for each strain