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Table 2 Carbon distribution of Kp(ptac-puuC + placiMALD) in a 5 L bioreactor (48 h)

From: Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis

Metabolites

3-HP

LA

AA

1,3-PDO

2,3-BDO

Total

Titer (mM)

298

7

163

84

7

861

Titer (g/L)

26.9

0.7

9.8

6.4

0.7

44.5

GCR (%)

34.6

0.8

18.9

9.8

0.8

64.9

  1. 3-HP 3-hydroxypropionic acid, LA lactic acid, AA acetic acid, 1,3-PDO 1,3-propanediol, 2,3-BDO 2,3-butanediol, GCR glycerol conversion ratio
  2. Kp(ptac-puuC + placiD), recombinant K. pneumoniae harboring vector overexpressing puuC and CRISPRi vector simultaneously targeting four lactate-producing enzyme genes ldhA, aldA, pmd and mgsA
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Microbial Cell Factories

ISSN: 1475-2859

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