Table 3 Plasmids and strains used in the present study
From: Synthesis of avenanthramides using engineered Escherichia coli
Plasmids or E. coli strain | Relevant properties or genetic marker | Source or reference |
|---|---|---|
Plasmids | ||
 pACYCDuet | P15A ori, Cmr | Novagen |
 pCDFDuet | CloDE13 ori, Strr | Novagen |
 pETDuet | f1 ori, Ampr | Novagen |
 pC-Os4CL-HCBT |  |  |
 pE-trpEG |  |  |
 pA-SeTAL | pACYCDuet carrying TAL from Saccharothrix espanaensis | [42] |
 pA-aroG-SeTAL-tyrA | pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from E. coli | [42] |
 pA-aroGfbr-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroGfbr, and tyrAfbr from E. coli | [42] |
 pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroGfbr, and ppsA, tktA, and tyrAfbr from E. coli | [42] |
 pG-HpaBC | pGEX 5X-3 carring HpaBC from E. coli | [30] |
 pG-SOMT9 | pGEX 5X-3 carring SOMT9 from soybean | [38] |
Strains | ||
 BL21 (DE3) | F− ompT hsdS B (r −B m −B ) gal dcm lon (DE3) | Novagen |
 BtyrR-trpD | BL21 (DE3) ΔtyrR/ΔtrpD | This study |
 HA-1 | BL21 harboring pC-Os4CL-HCBT |  |
 HA-2 | BL21 harboring pC-Os4CL-HCBT, pA-SeTAL, and pETDuet | This study |
 HA-3 | BL21 harboring pC-Os4CL-HCBT, pA-SeTAL, and pE-trpEG | This study |
 HA-4 | BL21 harboring pC-Os4CL-HCBT, pA-aroG-SeTAL-tyrA, and pE-trpEG | This study |
 HA-5 | BL21 harboring pC-Os4CL-HCBT, pA-aroGfbr-SeTAL-tyrAfbr, and pE-trpEG | This study |
 HA-6 | BL21 harboring pC-Os4CL-HCBT, pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr, and pE-trpEG | This study |
 HA-7 | BtyrR-trpD harboring pC-Os4CL-HCBT, pA-aroG-SeTAL-tyrA, and pE-trpEG | This study |
 HA-8 | BL21 harboring pC-Os4CL-HCBT, and pA-aroG-SeTAL-tyrA | This study |
 HA-Hpa | BL21 harboring pG-HpaaBC | This study |
 HA-S | BL21 harboring pG-SOMT9 | This study |