Skip to main content

Table 2 Catalytic constants on the analysed substrates

From: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Substrate

Km (mg/mL)

kcat (s−1)

kcat/Km (mg−1/s−1/mL)

Colloidal Chitin

1.7 ± 0.1

5 ± 0.1

3 ± 0.2

QS1

24 ± 12

8 ± 4

0.3 ± 0.2

QS2

2 ± 0.2

0.2 ± 0.02

0.1 ± 0.01

CHIT600

14 ± 4

0.08 ± 0.02

0.006 ± 0.002

CHIT100

9 ± 2

0.07 ± 0.02

0.008 ± 0.002

  1. Apparent Km and kcat values were obtained using chitosan as substrate. Values of kcat were calculated from Vmax considering a Chit42 protein molecular mass of 42 kDa
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us