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Table 1 Chit42 hydrolytic activity on the analysed substrates

From: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Substrate

MW (kDa)

DD (%)

Activity (%)

Colloidal Chitin

n.d.

≤ 5a

100 ± 6

QS1

98

81

29 ± 2

QS2

31

77

28 ± 2

CHIT600

600–00

> 90

2.3 ± 0.1

CHIT100

100–300

> 90

3.2 ± 0.2

  1. 100% activity: 0.2 U/mL
  2. Data are the average of 3 independent experiments. Standard errors were indicated
  3. n.d. not determined
  4. aDD of initial chitin flakes
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Microbial Cell Factories

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