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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Fig. 1

Activity profiles of cultures expressing Chit42. The P. pastoris transformant was grown in flask (a) and in fed-batch fermenter (b) supplemented with methanol. OD600 (black circles), pH (empty cycles) and extracellular chitinase activity using colloidal chitin as substrate (blue circles) were measured at the indicated times at 35 °C. Each point of activity represents the average of three independent measurements and standard errors are indicated

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