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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Fig. 1

SDS-PAGE analysis of recombinant protein lipBJ10 sample from BL21 (DE3) containing different recombinant plasmid from pET-SigPFL00 to pET-SigPFL06 induced with 0.2 mM IPTG for 40 h at 20 °C. 10 μg of total protein was loaded on a 12% SDS-PAGE gel. Lanes: M, molecular mass standard; 1, S(-)-lipBJ10; 2, DsbA-lipBJ10; 3, FhuD-lipBJ10; 4, MdoD-lipBJ10; 5, OmpA-lipBJ10; 6, YcdO-lipBJ10; 7, PelB-lipBJ10; a, uninduced whole-cell sonicated supernatant fraction; b, induced whole-cell sonicated supernatant fraction; c, periplasmic fraction; d, Inclusion bodies. Arrows indicate the position of lipBJ10 in the gel

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