Comprehensive subcellular topologies of polypeptides in Streptomyces

Table 1 Summary of previously existing subcellular annotation and comparison with SToPSdb

	Proteome annotation	Unit of measure	Uniprot	SToPSdb
S. lividans TK24 (total proteome)		Number of proteins	7322	7505
Subcellular topology annotations	Existing	Number or proteins	2153	7494
	Existing	% of reference proteome	29%	>99%
	Contradicting topology annotations between Uniprot and SToPSdb	Number or proteins	12	12
	Missing/unresolved	Number or proteins	5169	11
	Missing/unresolved	% of reference proteome	71%	< 1%

Approximately 30% of the S. lividans TK24 proteins contains proteome annotation for topology in Uniprot (2153 out of 7322). For 12 proteins Uniprot and SToPSdb were not in agreement. Protein topology for this strain was assigned de novo in SToPSdb leaving a small fraction of 11 proteins without topological annotation. Proteins annotated only in one of the two resources are labeled as “Unique (total)”

ISSN: 1475-2859