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Table 5 Alkaline phosphatase (PhoA) activity under conditions of AICAR overproduction

From: Specific features of l-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport

Strain

AICAr, mg/L

PhoA activity, µmol/min/mg

MG1655 (wild-type)

< 0.1

410 ± 7

MG1655ΔpurR::CmR ΔpurH::KmR

7.6

261 ± 1

  1. PhoA enzymatic activity was analyzed 2 h after Pi exhaustion. Average data of PhoA activity from 3 independent experiments are represented