Fig. 5

AP enzymatic activity under conditions of Pi limitation in His-producing and non-producing E. coli strains with different pitA alleles. MG1655, wild-type MG1655; MG1655 ∆pitA, MG1655 [ΔpitA::KmR]; KF37, MG1655+ [ΔpurR P his -ΔhisL′hisGE271KDCBHAFI]; KF37 ∆pitA, KF37 [ΔpitA:: KmR]. Average data from 3 independent experiments are represented, and error bars show the standard deviation (SD). PhoA enzymatic activity was analyzed 24 h after Pi exhaustion