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Table 1 The kinetic parameters of the wild type and mutants

From: A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys

Enzyme

Protein (μg/ml)

K m (mM)

K cat (s−1)

K cat /K m (s−1 mM−1)

Lip

203.41 ± 3.26

0.643 ± 0.03

9.8 ± 0.09

15.24 ± 0.21

V29A/Y193C

197.29 ± 2.54

0.363 ± 0.03

25.8 ± 0.16

71.08 ± 1.03

V29A

173.23 ± 2.17

0.624 ± 0.05

10.2 ± 0.11

16.35 ± 0.31

Y193C

183.42 ± 2.91

0.304 ± 0.04

26.1 ± 0.15

85.87 ± 1.62

  1. Data are given as mean values ± S.D. All the assays were performed at the optimal pH and temperature for the protein being studied
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Microbial Cell Factories

ISSN: 1475-2859

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