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Table 1 The kinetic parameters of the wild type and mutants

From: A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys

Enzyme Protein (μg/ml) K m (mM) K cat (s−1) K cat /K m (s−1 mM−1)
Lip 203.41 ± 3.26 0.643 ± 0.03 9.8 ± 0.09 15.24 ± 0.21
V29A/Y193C 197.29 ± 2.54 0.363 ± 0.03 25.8 ± 0.16 71.08 ± 1.03
V29A 173.23 ± 2.17 0.624 ± 0.05 10.2 ± 0.11 16.35 ± 0.31
Y193C 183.42 ± 2.91 0.304 ± 0.04 26.1 ± 0.15 85.87 ± 1.62
  1. Data are given as mean values ± S.D. All the assays were performed at the optimal pH and temperature for the protein being studied