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Table 2 Plasmids used in this study

From: Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae

Plasmid Description Source of reference
pGK423 Yeast expression vector containing PGK1 promoter, 2 μ origin, HIS3 marker, no expression (control plasmid) Ishii et al. [10]
pGK426 Yeast expression vector containing PGK1 promoter, 2 μ origin, URA3 marker, no expression (control plasmid) Ishii et al. [10]
pGK406 Yeast integration vector containing PGK1 promoter, URA3 maker Ishii et al. [10]
pATP425 Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, LEU2 marker, no expression (control plasmid)  
pATP424 Yeast three gene expression vector containing ADH1, TDH3 and PGK1 promoters, 2 μ origin, TRP1 marker, no expression (control plasmid)  
pATP423 Yeast three gene expression vector containing ADH1, TDH3 and PGK1 promoters, 2 μ origin, HIS3 marker, no expression (control plasmid)  
pGK423-kivd HIS3, expression of L. lactis kivd gene Kondo et al. [14]
pGK423-ARO10 HIS3, expression of S. cerevisiae ARO10 gene Kondo et al. [14]
pGK423-THI3 HIS3, expression of S. cerevisiae THI3 gene Kondo et al. [14]
pGK426-ADH1 URA3, expression of S. cerevisiae ADH1 gene Kondo et al. [14]
pGK426-ADH2 URA3, expression of S. cerevisiae ADH2 gene Kondo et al. [14]
pGK426-ADH5 URA3, expression of S. cerevisiae ADH5 gene Kondo et al. [14]
pGK426-ADH6 URA3, expression of S. cerevisiae ADH6 gene Kondo et al. [14]
pGK426-ADH7 URA3, expression of S. cerevisiae ADH7 gene Kondo et al. [14]
pGK426-SFA1 URA3, expression of S. cerevisiae SFA1 gene Kondo et al. [14]
pGK426-cimA URA3, expression of M. jannaschii cimA gene This study
pGK406-cimA URA3, genomic integration of M. jannaschii cimA gene This study
pATP425-leuC(Cb)-leuD(Cb) LEU2, co-expression of C. beijerinckii leuC and leuD genes This study
pATP425-leuC(Ec)-leuD(Ec) LEU2, co-expression of E. coli leuC and leuD genes This study
pATP425-leuC(Mj)-leuD(Mj) LEU2, co-expression of M. jannaschii leuC and leuD genes This study
pATP425-cimA-leuC(Cb)-leuD(Cb) LEU2, co-expression of M. jannaschii cimA, C. beijerinckii leuC and leuD genes This study
pATP425-cimA-leuC(Ec)-leuD(Ec) LEU2, co-expression of M. jannaschii cimA, E. coli leuC and leuD genes This study
pATP425-cimA-leuC(Mj)-leuD(Mj) LEU2, co-expression of M. jannaschii cimA, leuC and leuD genes This study
pATP424-ILV1 TRP1, expression of S. cerevisiae ILV1 gene This study
pATP424-tdcB TRP1, expression of E. coli tdcB gene This study
pATP424-ilvA TRP1, expression of E. coli ilvA gene This study
pATP424-tdcB-asd TRP1, co-expression of E. coli tdcB and asd genes This study
pATP423-thrA-thrB-thrC HIS3, co-expression of E. coli thrA, thrB and thrC genes This study
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Microbial Cell Factories

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