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Table 2 Plasmids used in this study

From: Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae

Plasmid

Description

Source of reference

pGK423

Yeast expression vector containing PGK1 promoter, 2 μ origin, HIS3 marker, no expression (control plasmid)

Ishii et al. [10]

pGK426

Yeast expression vector containing PGK1 promoter, 2 μ origin, URA3 marker, no expression (control plasmid)

Ishii et al. [10]

pGK406

Yeast integration vector containing PGK1 promoter, URA3 maker

Ishii et al. [10]

pATP425

Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, LEU2 marker, no expression (control plasmid)

 

pATP424

Yeast three gene expression vector containing ADH1, TDH3 and PGK1 promoters, 2 μ origin, TRP1 marker, no expression (control plasmid)

 

pATP423

Yeast three gene expression vector containing ADH1, TDH3 and PGK1 promoters, 2 μ origin, HIS3 marker, no expression (control plasmid)

 

pGK423-kivd

HIS3, expression of L. lactis kivd gene

Kondo et al. [14]

pGK423-ARO10

HIS3, expression of S. cerevisiae ARO10 gene

Kondo et al. [14]

pGK423-THI3

HIS3, expression of S. cerevisiae THI3 gene

Kondo et al. [14]

pGK426-ADH1

URA3, expression of S. cerevisiae ADH1 gene

Kondo et al. [14]

pGK426-ADH2

URA3, expression of S. cerevisiae ADH2 gene

Kondo et al. [14]

pGK426-ADH5

URA3, expression of S. cerevisiae ADH5 gene

Kondo et al. [14]

pGK426-ADH6

URA3, expression of S. cerevisiae ADH6 gene

Kondo et al. [14]

pGK426-ADH7

URA3, expression of S. cerevisiae ADH7 gene

Kondo et al. [14]

pGK426-SFA1

URA3, expression of S. cerevisiae SFA1 gene

Kondo et al. [14]

pGK426-cimA

URA3, expression of M. jannaschii cimA gene

This study

pGK406-cimA

URA3, genomic integration of M. jannaschii cimA gene

This study

pATP425-leuC(Cb)-leuD(Cb)

LEU2, co-expression of C. beijerinckii leuC and leuD genes

This study

pATP425-leuC(Ec)-leuD(Ec)

LEU2, co-expression of E. coli leuC and leuD genes

This study

pATP425-leuC(Mj)-leuD(Mj)

LEU2, co-expression of M. jannaschii leuC and leuD genes

This study

pATP425-cimA-leuC(Cb)-leuD(Cb)

LEU2, co-expression of M. jannaschii cimA, C. beijerinckii leuC and leuD genes

This study

pATP425-cimA-leuC(Ec)-leuD(Ec)

LEU2, co-expression of M. jannaschii cimA, E. coli leuC and leuD genes

This study

pATP425-cimA-leuC(Mj)-leuD(Mj)

LEU2, co-expression of M. jannaschii cimA, leuC and leuD genes

This study

pATP424-ILV1

TRP1, expression of S. cerevisiae ILV1 gene

This study

pATP424-tdcB

TRP1, expression of E. coli tdcB gene

This study

pATP424-ilvA

TRP1, expression of E. coli ilvA gene

This study

pATP424-tdcB-asd

TRP1, co-expression of E. coli tdcB and asd genes

This study

pATP423-thrA-thrB-thrC

HIS3, co-expression of E. coli thrA, thrB and thrC genes

This study