Table 1 Published alternative scaffold proteins and enzymes which have been engineered using yeast surface display
Engineered scaffold or enzyme | References |
|---|---|
Fn3 domain of fibronectin | Lipovsek et al. [24] Koide et al. [52] Hackel et al. [51] Koide et al. [40] Hackel et al. [55] Mann et al. [57] Sha et al. [58] Heinzelman et al. [59] Park et al. [49] |
Human kringle domains | Lee et al. [25] Lee et al. [66] |
DARPins | Schütz et al. [70] |
Cystine-knot miniproteins | Silverman et al. [74] Silverman et al. [75] Kimura et al. [76] Lahti et al. [77] Kimura et al. [78] Glotzbach et al. [79] Maaß et al. [80] |
Sso7d | Gera et al. [84] Gera et al. [85] Traxlmayr et al. [86] Kauke et al. [87] |
T7 phage gene 2 protein (Gp2) | Kruziki et al. [89] Chan et al. [90] |
Pyrazinamidase | Strauch et al. [91] |
vNAR domains | Zielonka et al. [26] Zielonka et al. [95] Könning et al. [27] Könning et al. [96] |
VHH domains | Ryckärd et al. [110] |
Variable lymphocyte receptor (VLR) | Tasumi et al. [14] Hong et al. [114] |
Horseradish peroxidase | Lipovsek et al. [122] Antipov et al. [123] |
Sortase A | Chen et al. [124] |
Lipases | Han et al. [125] White and Zegelbone [126] |
β-lactamase | Cohen-Khait and Schreiber [127] Cohen-Khait et al. [128] |
Glucose oxidase | Ostafe et al. [129] |
Nonribosomal Peptide Synthetase Adenylation Domain | Zhang et al. [130] |
Tobacco Etch Virus protease | Yi et al. [131] |