Fig. 4
Biochemical properties of TanBFnp. a Relative enzymatic activity of TanBFnp using methyl gallate as substrate over a range of pH. The observed maximum activity was defined as 100%. b Relative enzymatic activity of TanBFnp over a range of temperatures. The observed maximum activity was defined as 100%. c Relative enzymatic activity of TanBFnp after preincubation of the purified protein at 22, 30, 37, 45, 55 and 65 °C for the indicated lengths of time. d Absolute enzymatic activity of TanBFnp compared to previously described tannases using methyl gallate as substrate. The experiments were performed in triplicate. The values shown represent the mean value and standard error