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Table 3 Comparison of enzymatic characteristics among NFAmy13A and previously reported fungal α-amylases

From: Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter

Source fungi Specificity activity (U/mg) Optimal pH Optimal temperature (°C) Thermostability Inhibitor Activator Expression host References
Liquor starter (Fungus) 200.4 5–5.5 60 t1/2: 1.5 h at 60 °C, 0.4 h at 70 °C; in the presence of 0.01 M CaCl2, t1/2: 16 h at 60 °C, 0.7 h at 70 °C Co2+, Hg2+, SDS Ca2+, Mg2+ Pichia pastoris This study
Aspergillus awamori KT-11 78.9 4 50 ND Zn2+, Cu2+, Hg2+ Fe2+ Native [38]
Aspergillus flavus var. columnaris ND 5 50 96% loss of activity in absence of Ca2+, 10% loss of activity in presence of 0.01 M CaCl2 after incubation at 60 °C for 10 min None Ca2+ Native [43]
Aspergillus fischerianus 2314.1 4.5 50 t1/2: 17 min at 45 °C Cu2+ Co2+, Mn2+, Fe3+ Pichia pastoris [23]
Aspergillus niger CBS 513.88 2.2 5.5–6.5 NA Lost rapidly at above 35 °C for 10 min Na+ None Escherichia coli BL21 [21]
Aspergillus niger JGI 24 75 9 30 Around 20% loss of activity after incubation at 30 °C for 50 min Na+, Mg2+ Ca2+,Co2+ Native [32]
Aspergillus niveus ND 6.0 65 t1/2: 20 min at 70 °C Hg2+, Ag+, Fe3+ Ca2+, NH4+, Na+, K+, Mn2+, Mg2+ Native [33]
Aspergillus oryzae ATCC 76080 410 4–5 50 Lost 80% activity after incubation at 50 °C for 30 min Hg2+, DNFB, NBSI None Native [39]
Aspergillus oryzae EMS-18 1987.7 5.0 40 ND Mg2+, Mn2+, Na+, Zn2+, Ni2+, Fe2+, Cu2+, Co2+ and Ba2+ Ca2+ Native [44]
Aspergillus oryzae S2 5089.3, 6686.3 5.6 50 t1/2: 60 min at 50 °C Mg2+, Fe2+, Ba2+, Cu2+, Co2+, Mo2+, and Mn2+ Ca2+ Native [48]
Aspergillus tamarii 778.3 4.5–6.5 50–55 t1/2: 30 min at 70 °C Pb2+, Cu2+, Ag+, Fe3+ None Native [53]
Geomyces pannorum 12,800 5.0 40 40% loss of activity after incubation at 50 °C for 10 min Ni2+, EDTA, Methanol, Teween-20/80 Ca2+, Mg2+ Aspergillus oryzae [22]
Malbranchea cinnamomea S168 amylose 2240.7 6.5 65 Around 50% loss of activity after incubation at 60 °C for 30 min Hg2+, Cr3+ Mn2+, Co2+ Native [40]
Paecilomyces variotii 612.5 4 60 t1/2: 53 min at 60 °C Cu2+, Al3+Fe3+, EDTA, K+, Pb2+, Zn2+ Ca2+, Co2+, Mn2+ Native [34]
Pichia burtonii ND 4 40 20% loss of activity after incubation at 50 °C for 30 min Cd2+, Cu2+, Hg2+, Al3+, and Zn2+ None Native [41]
Preussia minima EL-14 138 9 25 ND Na+, Mg2+ Mn2+, Ca2+ Native [31]
Rhizopus oryzae 1123 4–6 60 50% loss of activity after incubation at 60 °C for 5 min Cu2+, Fe2+, Independence of Ca2+ Pichia pastoris [24]
Rhizomucor pusillus GX-3 20,732 5 70 t1/2: 75 min at 60 °C ND ND Pichia pastoris [25]
Saccharomycopsis fibuligera KZ, amylose 120–125 5–6 40–50 ND ND ND Saccharomyces cerevisiae [27]
Sclerotinia sclerotiorum ND 4 55 Around 80% loss of activity after incubation at 55 °C for 30 min Cu2+, Mn2+, Ba2+, Fe2+ Weakly stimulated by Ca2+ Native [45]
Scytalidium thermophilum 5.6 6.0 60 t1/2: 12 min at 60 °C Cu2+, Hg2+ Independence of Ca2+ Native [42]
Streptomyces megasporus 847.3 6 60 t1/2: 2 h at 70 °C Ag+, Ba2+, Co2+, Cu2+, EDTA, Hg2+, Mg2+, Pb2+, Zn2+ None Native [35]
Thermobifida fusca NTU22 ND 7 60 25% loss of activity after incubation at 60 °C for 3 h ND ND Pichia pastoris [26]
Thermomyces lanuginosus 349 5 70 32 and 94% loss of activity after incubation at 50 °C for 2 and 18 h Cu2+, FE3+ Ba2+, Ca2+, Cd2+, Mg2+, Mn2+ Native [46]
Thermomyces lanuginosus ND 5.6 65 At 65 °C (t1/2: 0.6 h) the enzyme is nearly 8 times more stable in presence of Ca2+ None Ca2+ Native [47]
Thermomyces lanuginosus ND 5.2 60 t1/2: 140 min at 60 °C, 10 min at 70 °C ND ND Native [36]
Talaromyces pinophilus 1–95 673.1 4.0–5.0 55 60% loss of activity after incubation at 55 °C for 1 h Ag+, Cu2+, Mn2+ Co2+, Fe2+, Fe3+ Native [49]
  1. ND, not determined