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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei

Fig. 3

Simultaneously deletion of two non-continuous large-scale genome regions in L. casei BL23. a Layout and genetic context of the ~ 12.8 kb genome region and the sizes of PCR products amplified with primers H1F and H2R from BLD3 strains. b Colony PCR results. M: DNA marker; 1–2: random picked chloramphenicol resistance colonies. c Growth of L. casei BL23 and the deletion strain L. casei BLD3 on MRS

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Microbial Cell Factories

ISSN: 1475-2859

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