Improved fatty aldehyde and wax ester production by overexpression of fatty acyl-CoA reductases

Table 2 The effect of the FAR overexpression on the WE production in different temperatures

T (°C)	Strain	CDW (g/l)	WE content^a (mg/g CDW)	WE titer^a (g/l)	Productivity^a (mg/(l h)	Yield^a (g/g glucose)
30	Control	5.6 ± 0.2	35 ± 2	0.20 ± 0.01	4.1 ± 0.3	0.014 ± 0.001
	W1 + Acr1	4.3 ± 0.5	93 ± 3	0.40 ± 0.06	8.4 ± 1.3	0.032 ± 0.003
	W1 + Ramo	5.3 ± 0.3	78 ± 8	0.41 ± 0.07	8.6 ± 1.4	0.025 ± 0.005
20	Control	4.0 ± 0.06	110 ± 0	0.43 ± 0.01	9.0 ± 0.2	0.035 ± 0.002
	W1 + Acr1	3.7 ± 0.02	125 ± 11	0.45 ± 0.04	9.4 ± 0.8	0.040 ± 0.002
	W1 + Ramo	4.1 ± 0.08	109 ± 5	0.45 ± 0.03	9.4 ± 0.6	0.040 ± 0.007

The W1 (ADP1Δ3381–3383::cm^r) cells expressing either Acr1 or Ramo under an IPTG-inducible promoter were cultivated at 30 and 20 °C for 48 h, the lipids extracted and wax esters measured with NMR. The average and standard deviation of two independent cultivations are shown. The control strain was ADP1Δ3381, which has the wild type Acr1 expression
CDW cell dry weight, WE wax ester
^aAn average molar mass of 506 g/mol for wax esters used in the calculations

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