Expression of cocoa genes in Saccharomyces cerevisiae improves cocoa butter production

Wei, Yongjun; Bergenholm, David; Gossing, Michael; Siewers, Verena; Nielsen, Jens

doi:10.1186/s12934-018-0866-2

Microbial Cell Factories

Table 1 List of strains used in this study

From: Expression of cocoa genes in Saccharomyces cerevisiae improves cocoa butter production

Strains	Parent strains	Expression plasmids	Properties
YJ0	CEN.PK 113-11C [18]	pBS01A	Empty plasmid pBS01A
YJ-G03	CEN.PK 113-11C	PYJ-G03	TcGPAT3 expression
YJ-G04	CEN.PK 113-11C	PYJ-G04	TcGPAT4 expression
YJ-G05	CEN.PK 113-11C	PYJ-G05	TcGPAT5 expression
YJ-G09	CEN.PK 113-11C	PYJ-G09	TcGPAT9 expression
YJ-G10	CEN.PK 113-11C	PYJ-G10	TcGPAT10 expression
YJ-G12-3	CEN.PK 113-11C	PYJ-G12	TcGPAT12 expression
YJ-G12-4	CEN.PK 113-11C	PYJ-G12-4	TcGPAT12-4 expression
YJ-L03	CEN.PK 113-11C	PYJ-L03	TcLPAT3 expression
YJ-L04	CEN.PK 113-11C	PYJ-L04	TcLPAT4 expression
YJ-L05	CEN.PK 113-11C	PYJ-L05	TcLPAT5 expression
SYJ0	CEN.PK 113-11C	pBS01A	Empty plasmid pBS01A, same as YJ0, but SYJ0 was constructed and tested at a different time compared to YJ0
SYJ-331	CEN.PK 113-11C	PYJ-331	TcGPAT3, TcLPAT3 and TcDGAT1 gene combination expression
SYJ-332	CEN.PK 113-11C	PYJ-332	TcGPAT3, TcLPAT3 and TcDGAT2 gene combination expression
SYJ-341	CEN.PK 113-11C	PYJ-341	TcGPAT3, TcLPAT4 and TcDGAT1 gene combination expression
SYJ-342	CEN.PK 113-11C	PYJ-342	TcGPAT3, TcLPAT4 and TcDGAT2 gene combination expression
SYJ-431	CEN.PK 113-11C	PYJ-431	TcGPAT4, TcLPAT3 and TcDGAT1 gene combination expression
SYJ-432	CEN.PK 113-11C	PYJ-432	TcGPAT4, TcLPAT3 and TcDGAT2 gene combination expression
SYJ-441	CEN.PK 113-11C	PYJ-441	TcGPAT4, TcLPAT4 and TcDGAT1 gene combination expression
SYJ-442	CEN.PK 113-11C	PYJ-442	TcGPAT4, TcLPAT4 and TcDGAT2 gene combination expression
Y29	IMX581 [19]	None	IMX581 sct1Δale1Δ lro1Δ dga1Δ
Y29-pBS01A	Y29	pBS01A	Empty plasmid pBS01A
Y29-TcD1	Y29	PYJ-TcDGAT1	TcDGAT1 expression
Y29-TcD2	Y29	PYJ-TcDGAT2	TcDGAT2 expression
Y29-331	Y29	PYJ-331	TcGPAT3, TcLPAT3 and TcDGAT1 gene combination expression
Y29-441	Y29	PYJ-441	TcGPAT4, TcLPAT4 and TcDGAT1 gene combination expression
YJW01	CEN.PK 113-11C	None	CEN.PK 113-11C sct1Δ
YJW02	CEN.PK 113-11C	None	CEN.PK 113-11C sct1Δ::LsGPAT
YJW09	CEN.PK 113-11C	None	CEN.PK 113-11C sct1Δ::LsGPAT gpt2Δ

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ISSN: 1475-2859

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