Fig. 1From: Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preferenceNeighbor-joining phylogenetic tree based on the amino acid sequences of DMWf18-543, DMWf18-558 and related lipolytic enzymes. Sequence alignment was performed using ClustalX, and the tree was constructed using MEGA software. Bootstrap values are based on 1000 replicates, and only values > 50% are shown. The scale bar indicates the number of amino acid substitutions per siteBack to article page