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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A feedback regulatory model for RifQ-mediated repression of rifamycin export in Amycolatopsis mediterranei

Fig. 2

Transcriptional analysis of rifP. a RT-PCR analysis of rifP in both S699 and ΔrifQ. rpoB was used as an internal control. b Characterization of the rifP transcriptional initiation site (TIS) using the primer extension assay. Primers designed for both rifP and rifQ were illustrated in the schematic chart, and the rifP TIS was indicated with a red vertical line in the chart and shown in red in the sequence. The primer extension result using rifP-pe2 was shown, and results with rifP-pe1 could be found in Additional file 6: Figure S6

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