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Table 1 Estimates of mutation rates (μ) in mutations/cell/generation for the MG1655 and MDS42 strains

From: The effect of metabolic stress on genome stability of a synthetic biology chassis Escherichia coli K12 strain

MDS42

24 (3.5)

168 (24.2)

336 (48.5)

504 (72.7)

cyc R

2.99·10−7

7.07·10−7

2.27·10−5

3.04·10−5

rif R

2.31·10−7

1.67·10−5

− 9.21·10−6*

1.51·10−6

Total

5.30·10−7

1.74·10−5

1.35·10−5

3.19·10−5

MG1655

24 (3.5)

168 (24.2)

336 (48.5)

504 (72.7)

cyc R

1.03·10−7

6.30·10−7

2.97·10−6

4.34·10−4

rif R

2.72·10−7

2.36·10−7

− 6.52·10−8*

5.99·10−4

Total

3.76·10−7

8.66·10−7

2.91·10−6

1.03·10−3

Fold diff. MDS42:MG1655

1.41

20.05

4.63

0.03

  1. Mutation rates for each strain (italics) were estimated separately at each locus, and then as a total. The time at which each sample was quantified is indicated in hours (italics) at the top of each column, with the number of generations, a scaled measure of time, indicated in the brackets below. The term ‘fold diff.’ refers to the ratio of the total mutation rate for MDS42 to MG1655 (μMDS42/μMG1655). The asterisks mark two negative mutation rate estimates. This occurred because rifR mutant colony counts for each strain during one out of three chemostat runs were slightly lower at 336 h compared to at 168 h, which produced a negative value
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Microbial Cell Factories

ISSN: 1475-2859

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