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Table 1 Estimates of mutation rates (μ) in mutations/cell/generation for the MG1655 and MDS42 strains

From: The effect of metabolic stress on genome stability of a synthetic biology chassis Escherichia coli K12 strain

MDS42 24 (3.5) 168 (24.2) 336 (48.5) 504 (72.7)
cyc R 2.99·10−7 7.07·10−7 2.27·10−5 3.04·10−5
rif R 2.31·10−7 1.67·10−5 − 9.21·10−6* 1.51·10−6
Total 5.30·10−7 1.74·10−5 1.35·10−5 3.19·10−5
MG1655 24 (3.5) 168 (24.2) 336 (48.5) 504 (72.7)
cyc R 1.03·10−7 6.30·10−7 2.97·10−6 4.34·10−4
rif R 2.72·10−7 2.36·10−7 − 6.52·10−8* 5.99·10−4
Total 3.76·10−7 8.66·10−7 2.91·10−6 1.03·10−3
Fold diff. MDS42:MG1655 1.41 20.05 4.63 0.03
  1. Mutation rates for each strain (italics) were estimated separately at each locus, and then as a total. The time at which each sample was quantified is indicated in hours (italics) at the top of each column, with the number of generations, a scaled measure of time, indicated in the brackets below. The term ‘fold diff.’ refers to the ratio of the total mutation rate for MDS42 to MG1655 (μMDS42MG1655). The asterisks mark two negative mutation rate estimates. This occurred because rifR mutant colony counts for each strain during one out of three chemostat runs were slightly lower at 336 h compared to at 168 h, which produced a negative value