Transformants Fatty acid | pYES2 (control) | pYES2-DsFADS6 | pYES2-TpFADS6 | ||||||
---|---|---|---|---|---|---|---|---|---|
+LA | +ALA | +LA and +ALA | +LA | +ALA | +LA and +ALA | +LA | +ALA | +LA and +ALA | |
16:0 (PA) | 22.9 ± 0.2 | 25.6 ± 0.2 | 20.5 ± 0.1 | 25.5 ± 0.4 | 21.9 ± 0.1 | 22.7 ± 0.3 | 23.4 ± 0.3 | 22.0 ± 0.6 | 24.0 ± 0.4 |
16:1 (PA) | 12.7 ± 0.3 | 14.6 ± 0.2 | 12.9 ± 0.3 | 14.4 ± 0.2 | 16.3 ± 0.3 | 11.8 ± 0.2 | 14.6 ± 0.2 | 14.4 ± 0.4 | 13.1 ± 0.6 |
18:0 (SA) | 11.4 ± 0.4 | 10.2 ± 0.6 | 11.8 ± 0.7 | 10.9 ± 0.4 | 12.5 ± 0.3 | 10.2 ± 0.4 | 12.0 ± 0.7 | 14.3 ± 0.5 | 9.3 ± 0.2 |
18:1 (OA) | 12.7 ± 0.8 | 10.3 ± 0.7 | 11.6 ± 0.4 | 12.5 ± 0.2 | 11.8 ± 0.6 | 12.6 ± 0.4 | 11.3 ± 0.4 | 11.2 ± 0.3 | 10.3 ± 0.4 |
18:2 (LA, ω-6) | 38.2 ± 0.3 | ND | 23.6 ± 0.1 | 29.6 ± 0.6 | ND | 16.1 ± 0.2 | 11.5 ± 0.5 | ND | 5.9 ± 0.6 |
18:3 (ALA, ω-3) | ND | 39.6 ± 0.5 | 21.2 ± 0.6 | ND | 37.5 ± 0.2 | 21.6 ± 0.3 | ND | 4.3 ± 0.6 | 2.7 ± 0.2 |
18:3 (GLA, ω-6) | ND | ND | ND | 7.2 ± 0.1 | ND | 5.0 ± 0.1 | 27.3 ± 0.3 | ND | 13.3 ± 0.3 |
18:4 (SDA, ω-3) | ND | ND | ND | ND | ND | ND | ND | 34.9 ± 0.4 | 16.7 ± 0.2 |
LA conversion ratea | – | – | – | 24.3 ± 1.3 | – | 23.8 ± 1.8 | 70.4 ± 0.2 | – | 69.3 ± 0.3 |
ALA conversion ratea | – | – | – | – | 0 | 0 | – | 89.0 ± 2.2 | 86.1 ± 1.3 |