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Table 1 Fatty acid compositions (% w/w) of the total lipid contents of yeast transformants harboring the control plasmid (pYES2) and the recombinant plasmids (pYES2-DsFADS6 and pYES2-TpFADS6)

From: Production of eicosapentaenoic acid by application of a delta-6 desaturase with the highest ALA catalytic activity in algae

Transformants

Fatty acid

pYES2 (control)

pYES2-DsFADS6

pYES2-TpFADS6

+LA

+ALA

+LA and +ALA

+LA

+ALA

+LA and +ALA

+LA

+ALA

+LA and +ALA

16:0 (PA)

22.9 ± 0.2

25.6 ± 0.2

20.5 ± 0.1

25.5 ± 0.4

21.9 ± 0.1

22.7 ± 0.3

23.4 ± 0.3

22.0 ± 0.6

24.0 ± 0.4

16:1 (PA)

12.7 ± 0.3

14.6 ± 0.2

12.9 ± 0.3

14.4 ± 0.2

16.3 ± 0.3

11.8 ± 0.2

14.6 ± 0.2

14.4 ± 0.4

13.1 ± 0.6

18:0 (SA)

11.4 ± 0.4

10.2 ± 0.6

11.8 ± 0.7

10.9 ± 0.4

12.5 ± 0.3

10.2 ± 0.4

12.0 ± 0.7

14.3 ± 0.5

9.3 ± 0.2

18:1 (OA)

12.7 ± 0.8

10.3 ± 0.7

11.6 ± 0.4

12.5 ± 0.2

11.8 ± 0.6

12.6 ± 0.4

11.3 ± 0.4

11.2 ± 0.3

10.3 ± 0.4

18:2 (LA, ω-6)

38.2 ± 0.3

ND

23.6 ± 0.1

29.6 ± 0.6

ND

16.1 ± 0.2

11.5 ± 0.5

ND

5.9 ± 0.6

18:3 (ALA, ω-3)

ND

39.6 ± 0.5

21.2 ± 0.6

ND

37.5 ± 0.2

21.6 ± 0.3

ND

4.3 ± 0.6

2.7 ± 0.2

18:3 (GLA, ω-6)

ND

ND

ND

7.2 ± 0.1

ND

5.0 ± 0.1

27.3 ± 0.3

ND

13.3 ± 0.3

18:4 (SDA, ω-3)

ND

ND

ND

ND

ND

ND

ND

34.9 ± 0.4

16.7 ± 0.2

LA conversion ratea

–

–

–

24.3 ± 1.3

–

23.8 ± 1.8

70.4 ± 0.2

–

69.3 ± 0.3

ALA conversion ratea

–

–

–

–

0

0

–

89.0 ± 2.2

86.1 ± 1.3

  1. ND not detected
  2. aConversion rate = 100 × ([product]/[product + substrate])