Fig. 8

The effect of different concentrations of PeSM on each alga growth and principal fatty acid profiles. DCW (a), TFA (b), LA level (c), ALA level (d), EPA level (e) and the percentage of EPA (TFA %) (f) were determined in the control strain (WT M. alpina) and transformants (including Ds-DsFADS6-9, Ds-DsFADS6-4, Ds-TpFADS6-8, and Ds-TpFADS6-10). Each alga was cultured in Ben-Amotz medium by supplementation with 500 mg/L MI, glucose/KNO₃ and 12% CO₂ aeration under varying temperature, containing PeSM-free, 1, 2 or 4 g/L PeSM as the exogenous substrate