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Table 2 (A) Random Forest results for the classification model, (B) Identification of an independent test dataset from unknown samples

From: Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level

Identified as

True

Lag

Log

Stationary

Specificity (%)

SDM

ANOSIM

(A)

 Lag

67

4

0

97.1

0.12

0.83 (lag, log)**

 Log

2

64

7

85.3

0.20

0.71 (log, stationary)**

 Stationary

0

7

63

90

0.15

0.89 (lag, stationary)**

 Sensitivity (%)

94.4

87.7

90

90.7

  

Group

No. of correctly assigned cells

No. of total cells

Accuracy (%)

Total accuracy (%)

(B)

 Lag

25

26

96.2

 

 Log

31

36

86.1

91.2

 Stationary

27

29

93.1

 
  1. The standard deviation of the means (SDM) of SCRS per sample was calculated (range from 0.12 to 0.20). Low SDM numbers represent high reproducibility and high reliability of the dataset. An analysis of similarity (ANOSIM) was performed to compare distances of between-group cells and within-group cells at different growth states. ** Represents p < 0.01. Lag, lag phase, log, log phase, stationary, stationary phase