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Table 2 Features and properties of target genes used in this study

From: A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli

Synthetic genes

Genbank/unioprot

Organism

Gene size (aa)

MW (kDa)

Cys (%)

GRAVY

Mycoplasma pneumonia community acquired respiratory distress syndrome toxin(CARDS TX)

AP017319.1

Mycoplasma pneumoniae

248

28.0

0.8

− 0.461

Vascular endothelial growth factors and receptors2 (VEGFR2)

EU826563.1

Homo sapiens

274

30.8

3.3

− 0.257

Rubella virus structural polyprotein

(RVs)

AB706305.1

Rubella virus

319

35.2

3.8

− 0.382

Outer membrane protein (Omp85)

LN847043.1

Chlamydia pneumoniae

253

27.8

0.0

− 0.397

Hirudin variant III (HV3)

KX215716.1

Hirudo orientalis

67

7.17

9.0

− 0.848