Fig. 5
From: A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
a Comparative expression of the target protein HV3. Key: expression of HV3 without any fusion tag (HV3); expression of HV3 with the Ffu312 tag (Ffu312-HV3); excretion to the culture medium (E); soluble fractions (S). b Purification of the fusion protein Ffu312-HV3. Key: supernatant of the fermentation broth (Lane 1); the purified protein Ffu312-HV3 (Lane 2). c SDS-PAGE analysis of fusion protein Ffu312-HV3 digested with enterokinase. Key: fusion protein Ffu312-HV3(Lane 1); fusion protein Ffu312-HV3 digested by EK (Lane 2). d Tricine/SDS-PAGE analysis of purified HV3. Key: purified HV3 (Lane 1)