Fig. 3
From: A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
a Expression of targeted proteins (i.e., CARDS TX, VEGFR-2, RVs, and Omp85) with the fusion tags Ffu209, Ffu217, Ffu312, MBP and NusA. The expression condition was 0.5 mM IPTG at 25 °C. Key: soluble fractions (S); inclusion bodies (ib). b Analysis of the expression of the Ffu fusion as compared direct expression, MBP fusions and NusA fusions. Total expression levels and solubility of the targeted proteins were calculated by Quantity One version 4.62 (Bio-Rad, USA) software, and was done three times